Janus kinases are non-receptor intracellular tyrosine kinases that play an important role in the pathogenesis of variant skin disorders and several hematological malignancies.<h4>Objective</h4>The aim of this study was to investigate the gene expression of Janus Kinase-1 (JAK1) and Janus Kinase-3 (JAK3) in the skin of different types of MF patients using Real-time Quantitative Reverse Transcription Polymerase Chain Reaction (RT-PCR).<h4>Methods</h4>The current study included 53 patients with MF, and 53 control samples. This evidence concerns the gene JAK3 and hematologic disorder.