However, the role and underlying molecular mechanisms of B4GALT1 in MASLD progression remain unclear.<h4>Methods and results</h4>The protein levels of B4GALT1 were elevated in patients with MASLD as well as in a murine model of MASLD induced by choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD), with a more pronounced increase observed in MASH. Here, B4GALT1 is linked to metabolic dysfunction-associated steatotic liver disease.