FISH assay was used to detect subcellular colocalization of hsa_circ_0000313 and miR-1224-3p.<h4>Results</h4>We observed that compared to healthy controls, MG patients exhibited increased expression of hsa_circ_0000313 and MKNK2, along with decreased expression of miR-1224-3p. Here, MKNK2 is linked to myasthenia gravis.