<h4>Background</h4>The purpose of this study is to explore the molecular mechanism of SIRT1 regulating chemotherapy resistance.<h4>Methods</h4>Expression level of SIRT1 in ovarian cancer cell lines SKOV3, SKOV3/DDP (cisplatin-resistant cell line) and normal ovarian epithelial cell line IOSE80 was detected. The gene discussed is SIRT1; the disease is ovarian carcinoma.