Moreover, our experimental validation confirmed that MRPS28 knockdown considerably suppressed proliferation, migration, and invasion, as well as promoted apoptosis in the breast cancer cell lines MDA-MB-231 and MCF-7.<h4>Conclusion</h4>Collectively, these findings imply that MRPS28 emerges as a potential diagnostic and prognostic biomarker across pan-cancer, with a potential oncogenic role in breast cancer progression. Here, MRPS28 is linked to cancer.