The role of MRPS28 in the malignant biological behavior of breast cancer cells was studied through <i>in vitro</i> experiments, including cell proliferation, migration, and invasion.<h4>Results</h4>Our analyses indicated that MRPS28 expression dysregulation was noted in various cancer types, and MRPS28 had remarkable diagnostic and prognostic predictive values. This evidence concerns the gene MRPS28 and breast carcinoma.