Deficiency of <i>PDIA4</i> in human umbilical vein endothelial cells suppressed functionality and inhibited the protein levels of both total and nuclear β-catenin as well as the downstream activity of the WNT/β-catenin signaling pathway.<h4>Conclusion</h4>Our study suggests that <i>PDIA4</i> may act as a susceptibility gene for CHD, and its deficiency may contribute to abnormal cardiac development by modulating the WNT/β-catenin signaling pathway. This evidence concerns the gene PDIA4 and coronary artery disorder.