Chemogenetic and optogenetic manipulations were applied to selectively modulate the mOFC<sup>CaMKIIα</sup>-MD and mOFC<sup>CaMKIIα</sup>-BLA pathways.<h4>Results</h4>The mOFC glutamatergic neurons are activated in neuropathic pain mice accompanied by anxiodepressive-like phenotypes. This evidence concerns the gene CAMK2A and Pain.