While lactoferrin (LTF) is a known multifunctional immunomodulatory glycoprotein, its precise role in modulating the NOX2-MPO-NETosis axis in ALI remains undefined.<h4>Methods</h4>We employed a time-course model of lipopolysaccharide (LPS)-induced ALI in C57BL/6N mice, combined with quantitative label-free lung proteomics and downstream bioinformatic analyses to map dynamic molecular changes. Here, LTF is linked to acute respiratory distress syndrome.