We identified that significant infiltration of Arg1<sup>+</sup>Sdc4<sup>+</sup>, Cxcl1<sup>+</sup>Ccl4<sup>+</sup>, and Mif<sup>+</sup>Cd63<sup>+</sup> macrophages may affect osteomyelitis through the Ccl3-Ccr1 and Cxcl2-Cxcr2 signaling pathways. Here, SDC4 is linked to osteomyelitis.