A dual-luciferase reporter assay was used to decipher the direct regulation of miR-10b-5p and miR-200b-3p on Occludin 3' UTR.<h4>Results</h4>Both short- and long-term antibiotic administration during the weaning period significantly alleviated DSS-induced colitis in male paternal mice, with long-term administration conferring a more pronounced protective effect. Here, OCLN is linked to colitis.