Bodyweight, hotplate latency, and uterine contractile amplitude and irregularity were measured.<h4>Main results and the role of chance</h4>Reduced PIEZO1 and eNOS staining and elevated PIEZO2 and OTR staining, concordant with the extent of fibrosis, were found in myometrium from women with adenomyosis, especially in myometrium proximal to adenomyotic lesions. Here, PIEZO1 is linked to adenomyosis.