Further, although we provided evidence that eNOS expression and NO production are determined by PIEZO1 expression levels, the precise mechanisms were not fully elucidated.<h4>Wider implications of the findings</h4>The stiffness-dependent increase in OTR and PIEZO2 expression, along with decreased PIEZO1 expression, underscores the progressive nature of adenomyosis. The gene discussed is NOS3; the disease is adenomyosis.