Dipeptidyl peptidase 4 (DPP4) or cluster of differentiation 26 (CD26) is involved in inflammation, infections, immune disorders, type 2 diabetes, kidney disease, and cancer.<h4>Methods</h4>We induced the steatosis phenotype in human induced pluripotent stem cell (iPSC)-derived hepatocyte-like cells (HLCs) by oleic acid (OA) feeding and confirmed regulation of clinically relevant pathways by NGS-based global transcriptomic analyses. The gene discussed is DPP4; the disease is type 2 diabetes mellitus.