The m6A demethylase ALKBH5 is highly expressed in various cancers, including AML, and promotes disease progression, but its specific role in modulating the PD-L1 axis and the immune microenvironment in AML remains unclear.<h4>Methods</h4>We analyzed the expression and prognostic significance of ALKBH5 in AML by integrating BloodSpot, GEO, and TCGA databases, and performed functional enrichment and immune infiltration analyses. The gene discussed is CD274; the disease is acute myeloid leukemia.