The sensitivity and specificity of mutant p53 expression for diagnosing ESCC were 1.00 (0.95-1.00) and 1.00 (0.95-1.00), respectively.<h4>Conclusion</h4>Mutant p53 expression can serve as an alternative marker for TP53 mutation screening used WES or WGS. Here, TP53 is linked to esophageal squamous cell carcinoma.