In this investigation, we identify AJUBA as an essential regulator that mediates the association between EMT and immune modulation in CRC.<h4>Methods</h4>By integrating multi-cohort transcriptomic datasets (The Cancer Genome Atlas (TCGA)-CRC, GSE18105, GSE22598, GSE89076, and GSE110224) with single-cell RNA-seq data (GSE132465), we applied machine learning and deep learning methodologies to comprehensively identify EMT-associated genes demonstrating prognostic significance. This evidence concerns the gene AJUBA and colorectal carcinoma.