NLRP3 and acute lung injury: <h4>Background</h4><i>Tetrastigma hemsleyanum</i> Diels et Gilg (Sanyeqing [SYQ]), a traditional anti-inflammatory herb, has been used to treat respiratory disorders.<h4>Aims</h4>To elucidate the mechanism of SYQ flavonoids in mitigating acute lung injury (ALI).<h4>Materials and methods</h4>An integrated approach combined network pharmacology, HPLC, lipopolysaccharide (LPS)-induced ALI mouse models, and NOD-like receptor thermal protein domain associated protein 3 (NLRP3)-activated cellular assays (LPS + nigericin).