We assessed whether the level of NEDD4L in these vesicles reflects NEDD4L expression in kidney biopsy samples.<h4>Methods</h4>Kidney biopsies (slides of control healthy kidney tissue taken adjacent to a suspected tumour site from five patients who had performed biopsy for suspicion of oncological pathology, and slides of kidney tissue from five patients with T2DM and DN) were utilised. The gene discussed is NEDD4L; the disease is type 2 diabetes mellitus.