In vivo and in vitro validations in LPS-induced AKI mice and HK-2 cells assessed histopathology, oxidative biomarkers, ferroptosis mediators, and key signaling pathways.<h4>Results</h4>Visnagin demonstrated affinity binding to <i>AKT1</i>, <i>NFE2L2</i>, <i>ACSL4</i>, and <i>TFRC</i>, indicating a potential role in modulating oxidative stress and ferroptosis. The gene discussed is NFE2L2; the disease is acute kidney injury.