Overexpression of GLS or <i>c-Myc</i> rescued the <i>CENPF</i> knockdown's inhibitory effect on GC cell growth.<h4>Conclusion</h4>Our findings identify a new <i>CENPF</i>/<i>c-Myc</i>/<i>GLS</i> axis that affects glutamine metabolism and cell survival in GC, implying that <i>CENPF</i> might be a novel target for the treatment of GC. The gene discussed is GLS; the disease is gastric cancer.