This study explores Centromere Protein F (CENPF)'s role in GC pathogenesis, specifically its regulation of glutamine metabolism.<h4>Methods</h4>The Cancer Genome Atlas-Stomach Adenocarcinoma (TCGA-STAD), GSE19826, and GSE27342 datasets were analyzed by bioinformatics to identify key candidate genes in GC. The gene discussed is CENPF; the disease is adenocarcinoma.