OSBPL3 and Patent ductus arteriosus: Inhibition of key enzymes involved in cholesterol synthesis resulted in a significant reduction in NOTCH pathway and stemness in PDA <i>in vivo</i> mouse models.<h4>Conclusions</h4>Aberrant expression of OSBPL3 plays a pivotal role in PDA initiation and progression and serves as an independent prognostic factor for poor outcomes in PDA patients.