<h4>Background</h4>This study analysed the relationship between serum chemokine CXC ligand 8 (CXCL8) and axillary lymph node metastasis (ALNM) in breast cancer (BC), evaluating its predictive value when combined with tumour markers and ultrasound imaging.<h4>Methods</h4>121 BC patients and 104 healthy controls were included, and serum CXCL8 was detected by enzyme-linked immunosorbent assay (ELISA) to compare the differences in the levels of CXCL8 and tumour markers in the two study groups. The gene discussed is CXCL8; the disease is breast cancer.