The analysis indicated that the most recent Hb value (Hb(t-1)) was the strongest predictor.<h4>Conclusion</h4>ML models based on demographic characteristics, dialysis records, and historical Hb data can effectively predict future Hb levels in MHD patients, which is helpful for early identification of anemia risk and timely clinical intervention. This evidence concerns the gene GSTM1 and anemia (phenotype).