This study systematically characterized the temporal and contextual roles of SOX9<sup>+</sup> hepatocytes during acute and chronic liver injury using integrated single-cell transcriptomics.<h4>Methods</h4>Publicly available single-cell RNA sequencing (scRNA-seq) datasets derived from mouse liver models of partial hepatectomy (PHx) and acetaminophen-induced acute liver injury (APAP), as well as human liver tissues with nonalcoholic fatty liver disease (NAFLD), were analyzed. Here, SOX9 is linked to metabolic dysfunction-associated steatotic liver disease.