scRNA-seq analysis identified Procollagen-Lysine,2-Oxoglutarate 5-Dioxygenase 1 (PLOD1) expression in CRC, Immunohistochemical staining (IHC) and Masson's trichrome staining were used to assess PLOD1 expression and collagen fiber content in CRC, its role in tumor invasion and migration was elucidated by wound healing and transwell assays.<h4>Results</h4>N1c samples exhibit enhanced extracellular matrix (ECM) remodeling and epithelial mesenchymal transition (EMT). Here, PLOD1 is linked to neoplasm.