To confirm this hypothesis, we investigated mixed HIV-1 infections in people who inject drugs (PWID) infected with the CRF01_AE subtype.<h4>Methods</h4>Viral plasma RNA from PWID were extracted, the V3 region of the HIV-1 gp120 gene was amplified, and deep sequencing was performed. The gene discussed is ITIH4; the disease is HIV-1 infection.