In this study, we aimed to dissect this interplay and to identify potential FOXO1-regulated lncRNAs which could possibly play a role in the pathogenesis of HCC.<h4>Methods</h4>This was achieved through the analysis of publicly available ChIP-Seq data provided by ENCODE database, along with performing RNA sequencing after the knockdown of FOXO1 in Huh-7 cells.<h4>Results</h4>ChIP-Seq data analyses revealed a list of 982 promising lncRNAs that are possibly regulated by FOXO1. This evidence concerns the gene FOXO1 and hepatocellular carcinoma.