IL6 and colitis: acidilactici</i> 46A significantly reduced colitis severity, preserved colonic mucosal architecture, and decreased inflammatory markers, including IL-1β and IL-6.<h4>Conclusion</h4><i>Pediococcus acidilactici</i> 46A is a safe and functionally active probiotic candidate that attenuates intestinal inflammation and preserves mucosal integrity in a murine model of colitis, supporting its potential application in the prevention or adjunctive treatment of inflammatory gut disorders.