Whole-genome sequencing revealed mutations in genes such as fhuA, purA, and rpoC, suggesting diverse resistance mechanisms linked to fitness trade-offs such as impaired biofilm formation.<h4>Conclusions</h4>Our findings highlight a precision-guided strategy with translational potential for device-associated infections, providing a mechanistic and methodological foundation for optimizing PAS-based therapies. The gene discussed is PURA; the disease is infection.