G6PD and Hyperbilirubinemia: This study aimed to determine the prevalence of G6PD gene variants among neonates at Johns Hopkins Aramco Healthcare and to evaluate their association with hyperbilirubinemia severity and phototherapy requirements.<h4>Methods</h4>We conducted a retrospective cohort study of neonates diagnosed with G6PD deficiency between January 2021 and December 2023.