Throughout the procedure, key physiological parameters were monitored and maintained stable.<h4>Results</h4>Our system successfully induced hallmark acute-phase responses to ECC, including a systemic inflammatory response (leukocytosis and elevated pro-inflammatory cytokines including IL-1β, IL-6, TNF-α, and IL-18), consumptive coagulopathy (thrombocytopenia), and metabolic stress (elevated lactate levels and electrolyte disturbances). The gene discussed is TNF; the disease is Increased total leukocyte count.