Silencing TPX2 reduced tumor volume and weight in nude mice, inhibited angiogenesis and proliferation, and suppressed EMT <i>in vivo</i>.<h4>Conclusion</h4>By activating the Wnt/β-catenin pathway, TPX2 promotes GC migration, invasion, and tumor angiogenesis, which collectively drive GC malignant progression. This evidence concerns the gene TPX2 and neoplasm.