IF confirmed lower level of SORBS2 expression in myofibroblast in the ESCC than those in the adjacent normal esophageal tissue.<h4>Conclusion</h4>We developed an ESCC diagnostic model and identified <i>BID</i>, <i>CBX3</i>, <i>ECT2</i>, <i>KIF14</i>, and <i>SORBS2</i> as robust ESCC biomarkers. Here, SORBS2 is linked to esophageal squamous cell carcinoma.