The roles of Notch1 signaling and OPN in NCF2-mediated macrophage polarization were evaluated using pharmacological inhibitors and neutralizing antibodies.<h4>Results</h4>NCF2 expression was significantly elevated in GBM tissues and cell lines and demonstrated a positive correlation with CD163+ M2 macrophage infiltration (r = 0.765, <i>p</i> < 0.001). Here, NOTCH1 is linked to glioblastoma.