Furthermore, integration of the detection of low-abundance TIGIT and OX40 by AuNP/PtNP tags and other 22 biomarkers by MCP tags, three significantly distinct T cell subpopulations in healthy donor and acute myeloid leukemia samples were identified, providing new targets and intervention strategies for the diagnosis and treatment of acute myeloid leukemia. Here, TIGIT is linked to acute myeloid leukemia.