This study aimed to elucidate the genetic causes of isolated FISH signals in lymphoma and myxoid liposarcoma samples, providing a more accurate basis for interpreting FISH results.<h4>Methods</h4>Six cases of lymphoma and myxoid liposarcoma, which showed isolated signals for <i>BCL2</i>, <i>MYC</i>, <i>BCL6</i>, or <i>DDIT3</i> in FISH detection, were carefully screened. The gene discussed is DDIT3; the disease is lymphoma.