NOS1 and ischemic stroke: Notably, R1400 and F1395, previously associated with the calmodulin-binding domain, influence conformational flexibility, while R1173 represents a novel interaction hotspot distinct from R1284.<h4>Discussion</h4>These results provide detailed insights into the dynamic mechanisms of the FNR-like unit and identify promising targets for the development of improved nNOS inhibitors to control excessive NO production in neurological disorders such as ischemic stroke.