<b>Materials and Methods:</b> Serum concentrations of PCT, IL-8, and Defb2 were measured using enzyme-linked immunosorbent assays (ELISA) in healthy control dogs (group 1, <i>n</i> = 40), dogs with superficial pyoderma (group 2a, <i>n</i> = 16), and dogs with deep pyoderma (group 3a, <i>n</i> = 7). This evidence concerns the gene CXCL8 and Pyoderma.