In addition, Western blot analysis demonstrated significantly increased GPX4 protein expression in retinal tissues from the rabbit PVR model compared with controls.<h4>Conclusion</h4>This study identifies TIMP1 and STAT3 as ferroptosis-associated candidate genes in proliferative vitreoretinopathy and highlights potential links among ferroptosis-related regulatory pathways, immune microenvironment alterations, and PVR pathogenesis. The gene discussed is GPX4; the disease is CAPN5-related vitreoretinopathy.