Pseudotime trajectory analysis delineated nine differentiation states, wherein CXCR4 expression persisted throughout the cellular differentiation trajectory.<h4>Conclusions</h4>This study identified CXCL12, CXCR4, GGT1, and VWF as key genes in COPD pathogenesis. Here, CXCR4 is linked to chronic obstructive pulmonary disease.