Western blotting, RT-qPCR and HPA database were utilized to further validate the role of NFE2L2, NNMT and GDF15 in CKD.<h4>Results</h4>By integrating bioinformatics including WGCNA, PPI, and GeneMANIA, 7 hub genes were identified including NNMT, GDF15, ACSL1, DLD, NFE2L2, PARP1, and NR4A1. Here, NR4A1 is linked to chronic kidney disease.