Within-group analyses, however, demonstrated a significant decrease in CEA in the IPC group [mean change: -2.23 ± 4.68 ng/mL (95% CI: 0.48-3.98); <i>P</i> = 0.014] and a significant increase in CA19-9 in the control group [mean change: +3.33 ± 7.07 U/mL (95% CI: 0.69-5.97); <i>P</i> = 0.015].<h4>Conclusions</h4>For patients with advanced CRC (T3/T4), laparoscopic radical resection combined with intraoperative raltitrexed IPC is feasible and exhibits an acceptable short-term safety profile. This evidence concerns the gene CEACAM5 and colorectal carcinoma.