Consistent with this, CLK1 was downregulated in NSCLC patient tissues of NSCLC, and its overexpression directly inhibits cancer cell proliferation <i>in vitro</i>.<h4>Conclusion</h4>Our findings demonstrate that CLK1 functions as a tumor suppressor gene in NSCLC, inhibiting proliferation and promoting immune infiltration. This evidence concerns the gene CLK1 and cancer.