The stability and function of Tregs are strongly influenced by the inflammatory microenvironment, yet the regulatory interactions between CD8<sup>+</sup> T cells and CD4<sup>+</sup>Foxp3<sup>+</sup> Tregs remain poorly understood.<h4>Methods</h4>We investigated the role of CD8<sup>+</sup> T cells in regulating induced Tregs (iTregs) using in vitro T cell co-culture assays and two in vivo models of autoimmune disease. Here, FOXP3 is linked to autoimmune disease.