Additionally, ovarian reserve indicators-including antral follicle count (AFC), basal serum levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), and E2, and anti-Müllerian hormone (AMH)-along with scores from the Self-Rating Anxiety Scale (SAS) will also be evaluated.<h4>Ethics and dissemination</h4>The trial has been approved by the ethics committees of all participating centers. Here, PLOD1 is linked to Anxiety.