This study investigates the therapeutic effects of <i>Anisakis simplex-</i>derived macrophage migration inhibitory factor (1) on adipogenesis, lipid metabolism, and inflammation in high-fat diet (2)-induced obesity.<h4>Methods</h4>We evaluated the effects of rAs-MIF on adipocyte differentiation, lipid droplet formation, and expression of adipogenic and inflammatory genes in 3T3-L1 cells. This evidence concerns the gene MIF and obesity disorder.