The results showed that 2',4'-DHC reduced the abundance of Proteobacteria, reshaped the bacterial diversity and composition.<h4>Discussion</h4>Overall, this study identified 2',4'-DHC in ACH that regulated the NLRP3 inflammasome activation to exert anti-inflammatory effects in IBD, highlighting its potential in treating NLRP3-related inflammatory diseases. The gene discussed is NLRP3; the disease is inflammatory bowel disease.