The positive control group showed severely altered lipid profile, liver enzymes, transforming growth factor-β (TGF-β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and liver histology, whereas treating the NAFLD with flaxseed oil or probiotic strains or their mix significantly improved lipid profile, liver enzyme, transforming growth factor-β (TGF-β), inflammatory markers (tumor necrosis factor-α (TNF-α) and interleukin-6 "IL-6"), and significantly decreased oxidative stress levels than the positive control group. The gene discussed is IL6; the disease is metabolic dysfunction-associated steatotic liver disease.