Markers of intestinal mucosal barrier function, including D-lactate and zonulin, decreased significantly (<i>p</i> < 0.01), while the level of milk fat globule-EGF factor 8 (MFG-E8) increased significantly (<i>p</i> < 0.01).<h4>Conclusion</h4>Tai Chi training can improve blood glucose homeostasis, gut microbiota richness and diversity, intestinal mucosal barrier function, and systemic inflammatory status in T2DM patients. This evidence concerns the gene HP and type 2 diabetes mellitus.