A look-up analysis of previously reported MD-associated genes in this cohort detected small but statistically significantly different methylation of CpGs located within collagen type XVIII alpha 1 (<i>COL18A1)</i> and peroxisome proliferator-activated receptor gamma, coactivator 1 beta (<i>PPARGC1B)</i> gene regions.<h4>Conclusions</h4>We provide preliminary evidence for modest methylation changes in specific genes associated with adherence to an MD. The gene discussed is PPARGC1B; the disease is Menkes disease.