However, DPN ameliorated the above Aβ-induced changes in cognitive behaviors, biomarkers, and histopathological levels.<h4>Conclusions</h4>Daphnetin attenuates Aβ-associated AD progression via inhibition of AChE activity, scavenging of free radicals, reduction of inflammation, and restoration of neuronal KCC2 channels. This evidence concerns the gene ACHE and Alzheimer disease.